Enzyme Technology
Heat treatment
In many cases, unwanted enzyme activities may be removed by
heat treatment. Different enzymes have differing susceptibility to heat
denaturation and precipitation. Where the enzyme required is relatively
heat-stable this allows its easy and rapid purification in terms of enzymic
activity. For such enzymes heat-treatment is always considered as an option at
an early stage in their purification. This method has been particularly
successfully applied to the production of glucose isomerase, where a short
incubation at a relatively high temperature is used (e.g., 60 - 85°C for
10 min). No interfering activity remains after this treatment and the
heat-treated, and hence leaky, cells may be immobilised and used
directly.
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This page was established in 2004 and last updated by Martin
Chaplin on
6 August, 2014
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