Enzyme Technology
Use of freeze-presses
The Hughes press and the 'X' press enable frozen
cell pastes to be forced under high pressure (150 - 230 MPa, 10 - 15 tons per
square inch) through narrow orifices, the disruption being produced by phase and
volume changes and by solid shear due to the ice crystals. The Hughes press can
only be used discontinuously on a small scale. The 'X' press may be used
semi-continuously and is amenable to scale-up but, although the method allows
the breakage of even the most robust organisms and the efficient recovery of
heat-sensitive enzymes, freeze-pressing is not used on a large scale for
releasing enzymes from cells.
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This page was established in 2004 and last updated by Martin
Chaplin on
6 August, 2014
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