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Enzyme Technology

Immobilised-enzyme processes

Immobilise -enzyme systems are used where they offer cost advantages to users on the basis of total manufacturing costs. The plant size needed for continuous processes is two orders of magnitude smaller than that required for batch processes using free enzymes. The capital costs are, therefore, considerably smaller and the plant may be prefabricated cheaply off-site Immobilised enzymes offer greatly increased productivity on an enzyme weight basis and also often provide process advantages (see Chapter 3) Currently used immobilised-enzyme processes are given in Table 5.1.


Table 5.1 Some of the more important industrial uses of immobilised enzymes

Enzyme

EC number

Product

Aminoacylase

3.5.1.14

L-Amino acids

Aspartate ammonia-lyase

4.3.1.1

L-Aspartic acid

Aspartate 4-decarboxylase

4.1.1.12

L-Alanine

Cyanidase

3.5.5.x

Formic acid (from waste cyanide)

Glucoamylase

3.2.1.3

D-Glucose

Glucose isomerase

5.3.1.5

High -fructose corn syrup

Histidine ammonia-lyase

4.3.1.3

Urocanic acid

Hydantoinase a

3.5.2.2

D- and L-amino acids

Invertase

3.2.1.26

Invert sugar

Lactase

3.2.1.23

Lactose-free milk and whey

Lipase

3.1.1.3

Cocoa butter substitutes

Nitrile hydratase

4.2.I.x

Acrylamide

Penicillin amidases

3.5.1.11

Penicillins

Raffinase

3.2.1.22

Raffinose-free solutions

Thermolysin

3.2.24.4

Aspartame

a Dihydropyrimidinase.



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This page was established in 2004 and last updated by Martin Chaplin
on 6 August, 2014